TCR Generierung

Verbesserung der Technologien zur Herstellung hochaffiner natürlicher TCRs mit erhöhter Empfindlichkeit und Sicherheit. Die Allo-Priming-Technologie liefert stärkere und nachhaltigere Immunantworten von TCRs, ohne dass eine Affinitätsreifung erforderlich ist. Standardisierungswerkzeuge wie Jovi Tag ermöglichen die Anreicherung von rekombinanten TCR (rTCR) -exprimierenden T-Zellen, um die Auswahl sicherer TCRs zu erleichtern.

TCR Generierung Publikationen

Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy
Kathrin Davari, Tristan Holland, Laura Prassmayer, Giulia Longinotti, Kenneth P Ganley, Lisa J Pechilis, Iulia Diaconu, Prashant R Nambiar, Michael S Magee, Dolores J Schendel, Daniel Sommermeyer, Christian Ellinger. Journal for ImmunoTherapy of Cancer; 9(3); 25 March 2021; doi: 10.1136/jitc-2020-002035
Identification of human T-cell receptors with optimal affinity to cancer antigens using antigen-negative humanized mice
Obenaus, M., Leitão, C., Leisegang, M., Chen, X., Gavvovidis, I., van der Bruggen, P., Uckert, W., Schendel, D.J. and Blankenstein, T. 2015. Nature Biotechnology 33, 402–407 (2015).
Mis-initiation of MART-1 transcription in the thymus and biased TCR usage can explain the high frequency of peripheral MART-1 specific T cells
Pinto, S., Sommermeyer, D., Michel, C., Wilde, S., Schendel, D, Uckert, W., Blankenstein T., Kyewski, B. 2014. Eur J Immunol. 2014 May 20 [Epub ahead of print].
Limitations for TCR gene therapy by MHC-restricted fratricide and TCR-mediated hematopoietic stem cell toxicity
Schendel, D.J. and Frankenberger, B. 2013. Oncoimmunology. 2013 Jan 1; 2(1):e22410.
Generation of allo-restricted peptide-specific T cells using RNA-pulsed dendritic cells: A three phase experimental procedure
Wilde, S., Geiger, C., Milosevic, S., Mosetter, B., Eichenlaub, S. and D.J. Schendel. 2012. Oncoimmunology. 1(2): 129-140.
Human antitumor CD8+ T cells producing Th1-polycytokines show superior antigen sensitivity and tumor recognition
Wilde, S., Sommermeyer, D., Leisegang, M., Frankenberger, B., Mosetter, B., Uckert, W. and D. J. Schendel. 2012. J Immunol. 189(2): 598-605.
High-quality and high-avidity T cell clones specific for tumor-targeted antigens and how to find them
Wilde, S. and D.J., Schendel. 2012. Oncoimmunology. 1(9):1643-1644.
Dendritic cells pulsed with RNA encoding allogeneic MHC and antigen induce T cells with superior anti-tumor activity and higher TCR functional avidity
Wilde, S., Sommermeyer, D., Frankenberger, B., Schiemann, M., Milosevic, S., Spranger, S., Pohla, H., Uckert, W., Busch, D.H. and D.J. Schendel. 2009. Blood 114(10): 2131-2139.

TCR Generierung Abstracts

AACR

T cells co-expressing a highly potent NY-ESO-1-specific TCR and a chimeric PD1-41BB co-stimulatory switch receptor show a favorable polyfunctional profile for the treatment of solid tumors Andrea Coluccio, Stefanie Tippmer, Kathrin Mutze, Petra U Prinz, Maja Buerdek, Barbara Loesch, Kathrin Davari, Giulia Longinotti, Dolores J Schendel

Poster

CAR-TCR Summit

Combined Molecular & Cellular Tools are Required to Assess Safety of TCR-T Immunotherapies; Tristan Holland

Präsentation

SITC Annual Meeting 2020

Development of a CD8 co-receptor independent T cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T cell-based immunotherapy; Davari K, Holland T, Prassmayer L, Longinotti G, Ganley K, Pechilis LJ, Diaconu I, Nambiar PR, Magee MS, Schendel DJ, Sommermeyer D and Ellinger C

Poster

CIMT – Annual Meeting of the Association for Cancer Immunotherapy 2019, Mainz, Germany

A safe and highly active NY-ESO-1/LAGE-1-specific TCR for adoptive T cell therapy of cancer; Longinotti G, Bürdek M, Weis M, Raffegerst S, Mösch A, Schendel DJ, Wehner C

Poster

AACR- American Association for Cancer Research Annual Meeting 2019, Atlanta

In vitro evaluation of TCR efficacy and toxicity using 3D spheroid models; Maja Buerdek, Kathrin Mutze, Kai Pinkernell, Dolores J. Schendel. Medigene Immunotherapies GmbH, Martinsried, Germany

Abstract

Poster

AACR – American Association for Cancer Research Annual Meeting 2017, Washington, D.C., USA

WT1 and PRAME mRNA transfected TLR 7/8-polarized fast DC vaccines in AML patients mount specific immune responses and impact progression free survival; Iris Bigalke, Guri Solum, Dag Josefsen, Yngvar Fløisand, Kirsti Hønnåshagen, Lisbeth Skoge, Signe Spetalen, Stein Sæbøe-Larssen, Dolores J. Schendel, Gunnar Kvalheim.

Abstract

Isolation and characterization of a PRAME-specific TCR with high avidity, potent antitumor efficacy and a favorable preclinical safety profile; Manon Weis, Carina Wehner, Christian Ellinger, Susanne Wilde, Dolores J. Schendel. Medigene Immunotherapies GmbH, Planegg/Martinsried, Germany

Abstract

SITC – Society for Immunotherapy of Cancer Annual Meeting 2014, National Harbor MD, USA

Wehner C., Ellinger C., Raffegerst S., Wilde S., Mosetter B., Weis M., Longinotti G., Sailer N., Schendel D.J., Milošević S. 2014. Generation of tumor antigen specific CD4+ and CD8+ T cells by simultaneous MHC-I and –II epitope presentation in vitro and in vivo. SITC National Harbor, USA.

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CIMT – Cancer Immunotherapy Annual Meeting 2013, Mainz

Ellinger C., Wehner C., Wilde S., Schendel D.J., Milošević S. Mai 2013. MHC Class-II Expression Targeting (CrossTAg) for the Generation of Tumor-Antigen-Specific CD4+ T Lymphocytes. CIMT Mainz, Germany.

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CIMT – Cancer Immunotherapy Annual Meeting 2013, Mainz

Wehner C., Ellinger C., Raffegerst S., Wilde S., Mosetter B., Schendel D.J., Milošević S., 2013. Isolation of antigen-specific CD8+ T lymphocytes and molecular characterization of antigenic epitopes. CIMT Mainz, Germany.

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Cellular Therapie International Symposium 2013, Erlangen  

Wilde S., Mosetter B., Wehner C., Ellinger C., Milosevic S., Frankenberger B., Schendel D. 2013. Isolation of NY-ESO-1-specific T cell receptors restricted by non-HLA-A*02:01 allotypes for TCR gene therapy. Cellular Therapie International Symposium Erlangen, Germany.

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